N myc interactor/NMI Antibody

• Catalog Number: orb251523
• Category: Antibodies
• Description: N myc interactor/NMI Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35057 MW
• UniProt ID: Q13287
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: N-myc-interactor;Nmi;N-myc and STAT interactor;NMI
• Note: For research use only
• Application notes: WB: The detection limit for NMI is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-NMI antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of NMI using anti-NMI antibody.Lane 1:human placenta tissue; 2:human K562 cell; 3:human Caco-2 cell; 4:human A431 cell;5:human PC-3 cell;6:human 293T cell.

IF analysis of NMI using anti-NMI antibody. NMI was detected in immunocytochemical section of A431 cells.

IHC analysis of NMI using anti-NMI antibody. NMI was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of NMI using anti-NMI antibody. NMI was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of NMI using anti-NMI antibody. NMI was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of NMI using anti-NMI antibody. NMI was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of NMI using anti-NMI antibody. NMI was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of NMI using anti-NMI antibody. NMI was detected in paraffin-embedded section of human placenta tissue.