MYC Antibody

• Catalog Number: orb1252119
• Category: Antibodies
• Description: MYC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MVH39-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A C-terminal peptide was used as the immunogen for the c-Myc antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the c-Myc antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MYC
• UniProt ID: P01106
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Myc proto-oncogene protein, Class E basic helix-lo
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the c-Myc antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human cervical carcinoma and c-Myc antibody (MVH39-1).

Western blot testing of HeLa cell lysate (nuclear fraction) with c-Myc antibody (clone MVH39-1). Expected molecular weight: 50~70kDa.