MSX1 Antibody

• Catalog Number: orb1265639
• Category: Antibodies
• Description: MSX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: MSX1
• UniProt ID: P28360
• NCBI: P28360
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Homeobox protein MSX-1, Homeobox protein Hox-7, Ms
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane.

Fluorescent image of U251 cell stained with MSX1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).MSX1 immunoreactivity is localized to Nucleus significantly.

Western blot analysis in A375 cell line lysates (35 ug/lane).

Western blot analysis in mouse spleen tissue lysates (35 ug/lane).

Fluorescent confocal image of SY5Y cells stained with MSX1 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MSX1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.