MSX1 Rabbit Polyclonal Antibody

SKU: orb2059

Description

MSX1 Rabbit Polyclonal Antibody

Research Area

Cell Markers, Cell Signaling, DNA and RNA Research, Epigenetics and Nuclear Signaling, Intercellular Signaling, Nervous System Development, Neurogenesis, Neuroscience, Transcription Factors

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=5μg /Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MSX1 (151-297/297aa)
• Target: MSX1
• Molecular Weight: 31 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ECTD3; HOX7; HYD1; STHAG1; Hox-7; Hox7.1; msh; MSX1_PANTR; MSX1; Msh homeobox 1-like protein; MSX1_BOVIN; MSX1_HUMAN; Homeobox protein Hox-7; MSX1_MOUSE; Hox-7.1; MSX1_MACMU;

Positive control: Hela cells, Concebtration: 5 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: Utreus (Mouse) Lysate at 40 ug, Hela Cell (Human) Lysate at 40 ug, Siha Cell (Human) Lysate at 40 ug, A673 Cell (Human) Lysate at 40 ug, Primary: Anti-MSX1 (orb2059) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37?? for 20 min, Incubation: Anti-MSX1 Polyclonal Antibody, Unconjugated (orb2059) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MSX1

UniProt

P28360

Avery Angell Swearer Spinal cord regeneration progresses via developmental and non-developmental mechanisms in X. tropicalis Dissertation, University of Washington, (2025)

PubMed:

Applications

IF

Reactivity

Zebrafish