MSX1 Rabbit Polyclonal Antibody

• Catalog Number: orb2059
• Category: Antibodies
• Description: MSX1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MSX1 (151-297/297aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=5μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: MSX1
• UniProt ID: P28360
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Msh homeobox 1 like protein; Homeobox 7; Homeobox
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Positive control: Hela cells, Concebtration: 5 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: Utreus (Mouse) Lysate at 40 ug, Hela Cell (Human) Lysate at 40 ug, Siha Cell (Human) Lysate at 40 ug, A673 Cell (Human) Lysate at 40 ug, Primary: Anti-MSX1 (orb2059) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37?? for 20 min, Incubation: Anti-MSX1 Polyclonal Antibody, Unconjugated (orb2059) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.