MSH6 Antibody

• Catalog Number: orb1261737
• Category: Antibodies
• Description: MSH6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Monkey, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human MSH6 (NP_000170.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 160kDa
• Target: MSH6
• UniProt ID: P52701
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: GTBP, GTMBP, HNPCC5, HSAP, p16DNA mismatch repair
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MSH6 antibody at 1:500 dilution.

Immunohistochemistry of paraffin-embedded human colon using MSH6 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using MSH6 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using MSH6 antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MSH6 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunofluorescence analysis of A549 cells using MSH6 antibody.