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Catalog Number | orb1151975 |
---|---|
Category | Antibodies |
Description | MSH6 Recombinant Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | M5F6 |
Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human MSH6 |
Antibody Type | Recombinant Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 153 kDa |
Target | MSH6 |
UniProt ID | P52701 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | DNA mismatch repair protein MSH6; G/T mismatch bin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:100, Primary incubation condition: 1 hour at room temperature, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1151975.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human SH-SY5Y cell lysates, Primary: Anti-MSH6 (orb1151975) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kDa, Observed band size: 180 kDa.
Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
Tissue: Human colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
Tissue: Mouse liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
Tissue: Rat testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Feline, Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |