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MSH6 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1151975

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1151975
CategoryAntibodies
DescriptionMSH6 Recombinant Rabbit Monoclonal Antibody
TargetMSH6
ClonalityRecombinant
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human MSH6
UniProt IDP52701
MW153 kDa
Tested applicationsICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
Antibody TypeRecombinant Antibody
Clone NumberM5F6
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesDNA mismatch repair protein MSH6; G/T mismatch bin
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NoteFor research use only
MSH6 Recombinant Rabbit Monoclonal Antibody

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:100, Primary incubation condition: 1 hour at room temperature, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1151975.

MSH6 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human SH-SY5Y cell lysates, Primary: Anti-MSH6 (orb1151975) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kDa, Observed band size: 180 kDa.

MSH6 Recombinant Rabbit Monoclonal Antibody

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.

MSH6 Recombinant Rabbit Monoclonal Antibody

Tissue: Human colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.

MSH6 Recombinant Rabbit Monoclonal Antibody

Tissue: Mouse liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.

MSH6 Recombinant Rabbit Monoclonal Antibody

Tissue: Rat testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.

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