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Catalog Number | orb1252060 |
---|---|
Category | Antibodies |
Description | MS4A1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CDLA20-1 |
Tested applications | FC, IF, IHC |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Recombinant CD20 protein was used as the immunogen for this CD20 antibody. The epitope is known to be extracellular. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD20 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | MS4A1 |
UniProt ID | P11836 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | B-lymphocyte antigen CD20, B-lymphocyte surface an Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD20 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of CD20 antibody and FFPE human tonsil tissue
IHC testing of CD20 antibody and human lymphoma tissue
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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