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Catalog Number | orb182382 |
---|---|
Category | Antibodies |
Description | CD20/MS4A1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106cells |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 33077 MW |
UniProt ID | P11836 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | B-lymphocyte antigen CD20;B-lymphocyte surface ant Read more... |
Note | For research use only |
Application notes | WB: The detection limit for CD20 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-CD20 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-CD20 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CD20 using anti-CD20 antibody.Lane 1:human Daudi cell.
IF analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of human B lymphocytic tumor tissues.
IHC analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of Human Tonsil Tissue.
IHC analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of Human Tonsil Tissue.
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