Mouse IL2 protein

• Catalog Number: orb80056
• Category: Proteins
• Description: IL2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17231 Dalton. The IL-2 is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: mIL 2
• Solubility (25°C): It is recommended to reconstitute the lyophilized Mouse IL-2 in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ.
• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine CTLL-2C ells is 0.18 ng/ml.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein (1mg/ml) was lyophilized extensive dialysis against 10mM sodium citrate buffer to pH=4.
• Alternative names: Interleukin-2 protein, T-cell growth factor (TCGF)
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-2 as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Mouse IL2 protein