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Mouse IL2 protein

Catalog Number: orb80056

DispatchUsually dispatched within 2-3 weeks
$ 170.00
Catalog Numberorb80056
CategoryProteins
DescriptionIL2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17231 Dalton. The IL-2 is purified by proprietary chromatographic techniques.
TargetmIL 2
ConjugationUnconjugated
Buffer/PreservativesThe protein (1mg/ml) was lyophilized extensive dialysis against 10mM sodium citrate buffer to pH=4.
Purity> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequencePTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ.
Tested applicationsFA, HPLC, SDS-PAGE
Application notes< strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-2 as Reference Standard.
Biological OriginE.coli
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of murine CTLL-2C ells is 0.18 ng/ml.
Solubility (25°C)It is recommended to reconstitute the lyophilized Mouse IL-2 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Alternative namesInterleukin-2 protein, T-cell growth factor (TCGF)
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NoteFor research use only
Mouse IL2 protein

SDS-PAGE analysis of Mouse IL2 protein

  • Goat anti-ITK Antibody [orb18315]

    ELISA,  IF,  WB

    Human, Mouse

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Mouse IL2 protein (Active) [orb359008]

    > 95% as determined by SDS-PAGE and HPLC.

    17.2 kDa

    E.Coli

    500 μg, 5 μg, 100 μg
  • Mouse Il2 protein [orb594836]

    Greater than 95% as determined by SDS-PAGE.

    17.4 kDa

    E.coli

    1 mg, 500 μg, 10 μg, 50 μg
  • Anti-AREB6 Antibody [orb1473904]

    FC,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • Mouse IL2RA Protein, hFc Tag [orb1290855]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of mIL2RA-hFc is approximately 70-100 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg