Mouse IgG1 antibody

• Catalog Number: orb347364
• Category: Antibodies
• Description: Mouse IgG1 antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG1 heavy chain
• Concentration: 1.20 mg/mL
• Dilution range: ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG2a, IgG2b and IgG3 reactivity was less than 5% against target (IgG1, IgG). Cross-reactivity against Bovine is assessed only by IEP.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-mouse IgG1 antibody, goat anti-mouse IgG
• Note: For research use only
• Application notes: Anti-Mouse IgG1 secondary antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
• Expiration Date: 12 months from date of receipt.