GIT1 Antibody

• Catalog Number: orb99082
• Category: Antibodies
• Description: Mouse Anti-Rat GIT1 Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N39B/8 (Formerly sold as S39B-8)
• Tested applications: ICC, IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Unconjugated
• MW: 90kDa
• Target: GIT1
• Entrez: 83709
• UniProt ID: Q9Z272
• NCBI: NP_114002.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: ARF GAP GIT1 Antibody, ARF GTPase-activating prote
• Note: For research use only
• Application notes: 1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

References

Cason, Sydney E. et al. Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases bioRxiv, (2023)

PubMed: 36747648

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone N39B/8. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone N39B/8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.