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Mouse CD274 protein
Catalog Number: orb391394
| Catalog Number | orb391394 |
|---|---|
| Category | Proteins |
| Description | Recombinant mouse CD274 protein is produced by our mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a 6His tag at the C-terminus. |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Reactivity | Mouse |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Target | CD274 |
| Entrez | 60533 |
| UniProt ID | Q9EP73 |
| Protein Sequence | FTITAPKDLY VVEYGSNVTM ECRFPVEREL DLLALVVYWE KEDEQVIQFV AGEEDLKPQH SNFRGRASLP KDQLLKGNAA LQITDVKLQD AGVYCCIISY GGADYKRITL KVNAPYRKIN QRISVDPATS EHELICQAEG YPEAEVIWTN SDHQPVSGKR SVTTSRTEGM LLNVTSSLRV NATANDVFYC TFWRSQPGQN HTAELIIPEL PATHPPQNRT VDHHHHHH |
| Source | Human cells |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Alternative names | B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell dea Read more... |
| Note | For research use only |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
Immobilized Anti-CD274 antibody at 2 μg/ml (100 μl/well) can bind Recombinant Mouse CD274 Protein. The ED50 of Recombinant Mouse CD274 Protein is 0.96 μg/ml.
- Item 1 of 4
Human PD-L1 Protein, mFc-His tag [orb689384]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 2
Mouse PD-L1 Protein, Fc Tag [orb257764]
Unconjugated
95%
51.4 kDa
Mouse PD-L1, Fc Tag (orb257764) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693).
200 μg, 1 mg - Item 1 of 1
Mouse CD274 protein [orb391395]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
10 μg, 50 μg, 500 μg - Item 1 of 2
Recombinant mouse PD-L1 protein, C-hFc (HEK293) [orb1516347]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
50 μg - Item 1 of 2
Recombinant mouse PD-L1 protein, C-His (HEK293) [orb1516348]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
50 μg
