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Description
Research Area
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human PD-L1-mFc(Cat#orb2994385) at 2μg/ml (100 μl/well)can bind Anti-Human PDL1 mAb-Fc(Cat#orb2993144).The ED50 of Anti-Human PDL1 mAb-Fc(Cat#orb2993144) is 4.09 ng/ml. |
| Tag | C-mFc |
| Expression Region | Phe19-Thr239 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 50.9 KDa. Observed: 59-89 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CD274/PD-L1/B7-H1 Human Recombinant Antibody [orb2958447]
ELISA, FC, WB
Human
Human
Monoclonal
Unconjugated
1 mg, 50 μg, 100 μgRecombinant human PD-L1 protein, C-His (HEK293) [orb1516352]
>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.
100 μgHuman PD-L1 Protein, mFc-His tag [orb689384]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Mammalian
100 μg, 50 μg, 10 μg

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