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Catalog Number | orb1671637 |
---|---|
Category | Antibodies |
Description | MME Antibody |
Clonality | Recombinant |
Clone Number | FR4D11 |
Tested applications | FC, IF, IHC |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | This antibody was raised by immunizing mice with Raji cells. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | MME |
UniProt ID | P08473 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Neprilysin, Atriopeptidase, Common acute lymphocyt Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot using anti-CD10 antibody FR4D11. Daudi cell lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of FR4D11 (orb1671637) at 2 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of fixed Daudi cells with anti-CD10 antibody FR4D11. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of FR4D11 (orb1671637) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671637- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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