MMP12 Antibody

• Catalog Number: orb1263950
• Category: Antibodies
• Description: MMP12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: MMP12
• UniProt ID: P39900
• NCBI: P39900
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Macrophage metalloelastase, MME, Macrophage elasta
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in 293 cell line lysate.

Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse kidney tissue lysates.

Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).