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Catalog Number | orb182390 |
---|---|
Category | Antibodies |
Description | CD10/MME Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 85514 MW |
UniProt ID | P08473 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Neprilysin;3.4.24.11 ;Atriopeptidase;Common acute Read more... |
Note | For research use only |
Application notes | WB: The detection limit for CD10 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of Daudi cells using anti-CD10/MME antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of CD10/MME using anti-CD10/MME antibody.Lane 1:human Daudi cell;2:human U-87MG cell;3:human placenta tissue;4:rat kidney tissue.
IHC analysis of CD10/MME using anti-CD10/MME antibody. CD10/MME was detected in a paraffin-embedded section of human lymphoma tissue.
IHC analysis of CD10/MME using anti-CD10/MME antibody. CD10/MME was detected in a paraffin-embedded section of human placenta tissue.
IHC analysis of CD10/MME using anti-CD10/MME antibody. CD10/MME was detected in a paraffin-embedded section of human renal cell carcinoma tissue.
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