MLH1 Antibody / MutL Homolog 1

• Catalog Number: orb1151674
• Category: Antibodies
• Description: Mouse monoclonal antibody to MLH1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MLH1/6467
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human MLH1 protein was used as the immunogen for the MLH1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This MLH1 antibody is available for research use only.
• UniProt ID: P40692
• Storage: Aliquot the MLH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MLH1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free MLH1 antibody (clone MLH1/6467) as confirmation of integrity and purity.

IHC staining of FFPE human ovarian carcinoma tissue with MLH1 antibody (clone MLH1/6467). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE Lynch Syndrome / Hereditary Non-Polyposis Colorectal Cancer tissue (HNPCC) with MLH1 antibody (clone MLH1/6467) at 2 ug/ml in PBS, 30 min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.