Milk Fat Globule Antibody (MFG-E8, Lactadherin)

• Catalog Number: orb749666
• Category: Antibodies
• Description: Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.
• UniProt ID: Q08431
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM291
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This MFG-E8 antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFG-E8 antibody (SPM291)

IHC: FFPE human renal cell carcinoma tested with MFG-E8 antibody (SPM291)

IHC: FFPE human pancreas tested with MFG-E8 antibody (SPM291)

IHC: FFPE human lung carcinoma tested with MFG-E8 antibody (SPM291)