Milk Fat Globule Antibody (MFG-E8, Lactadherin)

• Catalog Number: orb749667
• Category: Antibodies
• Description: Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-EA. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A delipidated human milk fat globule membrane preparation was used as the immunogen for the Lactadherin antibody.
• UniProt ID: Q08431
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the MFG-E8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: EDM45
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Lactadherin antibody is available for research use only.
• Note: For research use only

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with Lactadherin antibody (EDM45)

IHC analysis of FFPE human lung carcinoma tested with Lactadherin antibody (EDM45)

IHC analysis of FFPE human renal cell carcinoma tested with Lactadherin antibody (EDM45)

IHC analysis of FFPE human pancreas tested with Lactadherin antibody (EDM45)

Western blot testing of MCF7 cell lysate with Lactadherin antibody (clone EDM45).