MHC II antibody

• Catalog Number: orb758780
• Category: Antibodies
• Description: Rat monoclonal antibody to MHC II
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: P7/7
• Tested applications: FC, IHC, IP
• Reactivity: Mouse, Rat
• Isotype: IgG2b
• Immunogen: This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: MHC II
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: P7.7; I-A; Ia; I-E; MHC-II; MHC Class II
• Note: For research use only
• Application notes: This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987; Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004; Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).
• Expiration Date: 12 months from date of receipt.