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    HLA-DR Antibody

    Catalog Number: orb1520114

    DispatchUsually dispatched within 5-10 working days
    $ 230.00
    Catalog Numberorb1520114
    CategoryAntibodies
    DescriptionHLA-DR Antibody
    Species/HostMouse
    ClonalityMonoclonal
    Tested applicationsFC, ICC, IHC, WB
    ReactivityHuman
    ImmunogenActivated human peripheral blood mononuclear cells
    Concentration50 µg/ml
    Dilution rangeWB - 1:1000; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
    Form/AppearanceWhole IgG
    ConjugationUnconjugated
    TargetHLA-DR
    UniProt IDP01903
    NCBINP_061984.2
    Storage2 - 8°C
    Buffer/PreservativesPhosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
    Alternative namesMHC class II antigen DRA; HLA-DRA1; histocompatibi
    Read more...
    NoteFor research use only
    Application notesFormat: Whole IgG
    Expiration Date12 months from date of receipt.
    HLA-DR Antibody

    Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 µg) from KG-1, SNB-75, Raji, Ramos, and SR (10 µg) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

    HLA-DR Antibody

    Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3]. Secondary: HRP-conjugated goat anti-mouse IgG.

    HLA-DR Antibody

    Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG.

    HLA-DR Antibody

    Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114). Secondary: HRP-conjugated goat anti-mouse IgG.

    HLA-DR Antibody

    Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (orb1520114) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG.

    HLA-DR Antibody

    Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™. Counterstain: DAPI (blue).

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