RFX1 Antibody

• Catalog Number: orb1525559
• Category: Antibodies
• Description: RFX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 929 and 979
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RFX1
• UniProt ID: P22670
• NCBI: NP_002909.4
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: MHC class II regulatory factor RFX; transcription
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RFX1 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, MCF-7, HEK293T, K-562, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (orb1525559) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody (orb1525559) used for IP at 6 µg per reaction. RFX1 was also immunoprecipitated by a previous lot of this antibody (orb1525559) and a second antibody against a different epitope of RFX1.

Detection of human RFX1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. orb1525559) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse RFX1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. orb1525559) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of mouse RFX1 by western blot. Samples: Whole cell lysate (25 µg) from 3T3-L1, CT26, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (orb1525559) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.