MHC Class II antibody

• Catalog Number: orb154373
• Category: Antibodies
• Description: Rat monoclonal antibody to MHC Class II which belongs to the MHC class II family that is expressed by antigen presenting cells including B cells, dendritic cells and macrophages. The main function of major histocompatibility complex (MHC) class II molecules is to present processed antigens, which are derived primarily from exogenous sources, to CD4(+) T-lymphocytes. MHC class II molecules thereby are critical for the initiation of the antigen-specific immune response.
• Clonality: Monoclonal
• Clone Number: M5/114
• Tested applications: FA, FC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Mouse
• Isotype: Rat IgG2b
• Immunogen: Activated C57BL/6 mouse spleen cells
• Concentration: 1 mg/ml
• Dilution range: Functional application: Blocking of T cell proliferative responses. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
• Purity: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Target: MHC Class II
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Note: For research use only
• Application notes: Functional application: Blocking of T cell proliferative responses. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.