MGMT Antibody

• Catalog Number: orb234312
• Category: Antibodies
• Description: MGMT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 21646 MW
• UniProt ID: P16455
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Methylated-DNA--protein-cysteine methyltransferase
• Note: For research use only
• Application notes: WB: The detection limit for MGMT is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of MGMT using anti-MGMT antibody.MGMT was detected in immunocytochemical section of human MCF-7 cell.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

Flow Cytometry analysis of THP-1 cells using anti-MGMT antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MGMT using anti-MGMT antibody.Lane 1:human Jurkat cell; 2:human MDA-MB-453 cell.

IF analysis of MGMT using anti-MGMT antibody. MGMT was detected in immunocytochemical section of MCF7 cells.

IF analysis of MGMT using anti- MGMT antibody. MGMT was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of MGMT using anti-MGMT antibody. MGMT was detected in a paraffin-embedded section of Human Placenta tissue.