MGMT antibody

• Catalog Number: orb1473978
• Category: Antibodies
• Description: Mouse monoclonal antibody to MGMT
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human MGMT. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/10 - 1/50), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MGMT
• Entrez: 4255
• UniProt ID: P16455
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Methylated-DNA--protein-cysteine methyltransferase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MGMT expression in MCF7 (A) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 25 kD)

Immunohistochemical analysis of MGMT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MGMT staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).