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Catalog Number | orb700631 |
---|---|
Category | Proteins |
Description | The MERS or Middle East Respiratory Syndrome Coronavirus, is a member of the coronavirus family and is known to cause severe respiratory illness in humans. The MERS protein is a type I transmembrane protein that plays a vital role in the virus's ability to infect host cells. It is composed of three main domains: the extracellular domain, the transmembrane domain, and the cytoplasmic domain. The extracellular domain, further divided into S1 and S2 subunits, is responsible for receptor recognition and binding. The S1 subunit contains the Receptor Binding Domain (RBD), which is crucial for the virus's attachment to and entry into host cells. The RBD specifically interacts with the host cell receptor, facilitating the initial step of infection. The S2 subunit, on the other hand, is responsible for membrane fusion. It contains a fusion peptide that inserts into the host cell membrane, initiating the fusion process. This fusion allows the virus's genetic material to be released into the host cell, initiating the infection process. |
Reactivity | Virus |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 83.1 kDa |
Target | Spike S1 |
Protein Sequence | Tyr 18 - Arg 751 |
Source | MERS S1 protein, His Tag (orb700631) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Arg 751 (Accession # K0BRG7-1). |
Expression System | HEK293 |
Expression Region | Tyr 18 - Arg 751 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Spike,S1 protein,Spike glycoprotein Subunit1,S gly Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.1 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
83.1 kDa | |
HEK293 cells |
> 95% pure as determined by 12% PAGE (coomassie staining). | |
E. coli |
> 95% by SDS-PAGE. | |
KMP1877, Recombinant MERS-CoV Spike RBD Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Glu367-Tyr606) of mers-cov Spike RBD (Accession #AFS88936.1) fused with an 6×His Tag at the C-terminus. |
> 95% by SDS-PAGE. | |
KMP1871, Recombinant MERS-CoV Spike S1 Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Tyr18-Glu725) of mers-cov Spike S1 (Accession #AFS88936.1) fused with an 6×His Tag at the C-terminus. |
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