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Catalog Number | orb1231501 |
---|---|
Category | Proteins |
Description | MERS S1 Recombinant Protein |
Tag | His Tag |
Form/Appearance | Lyophilized |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | > 95% as determined by SDS-PAGE. |
Protein Sequence | Tyr 18 - Arg 751 |
MW | 83.1 kDa |
Tested applications | ELISA, WB |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.1 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Source | HEK293 cells |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Spike,S1 protein,Spike glycoprotein Subunit1,S gly Read more... |
Note | For research use only |
MERS S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human DPPIV, Fc Tag at 2 ug/ml (100 uL/well) can bind MERS S1 protein, His Tag with a linear range of 0.039-0.156 ug/ml.
98.00% | |
142.52 kDa (predicted); 157.6 kDa (reducing conditions) |
Greater than 85.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
93.30% | |
79.9 kDa (predicted); 94 kDa (reducing condition, due to glycosylation) |
82.90% | |
44.10 kDa (predicted) |