MeCP2 Antibody

• Catalog Number: orb1931563
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25959
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:2000, IHC-P: 1:500, IHC-P: 1:200
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 52441 Da
• Target: This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from human MeCP2.
• UniProt ID: P51608
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining MeCP2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining MeCP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/200) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-MeCP2 (S423) Antibody at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MeCP2 Antibody at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysates. Lane 2: Jurkat whole cell lysates. Lane 3: A431 whole cell lysates. Lane 4: HepG2 whole cell lysates. Lane 5: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.