MCP1 Rabbit Polyclonal Antibody

• Catalog Number: orb704179
• Category: Antibodies
• Description: MCP1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Recombinant rat MCP-1 full length
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: CCL2
• UniProt ID: P14844
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MCP-1/CCL2; C-C motif chemokine 2; CCL 2; CCL2; CC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MCP1) Polyclonal Antibody, Unconjugated (orb704179) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Lung (Rat) Lysate at 40 ug, Lane 2: Spleen (Rat) Lysate at 40 ug, Primary: Anti-MCP1 (orb704179) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14/11 kD, Observed band size: 11 kD.

Sample: Lane 1: Lung (Rat) Lysate at 40 ug, Lane 2: Spleen (Rat) Lysate at 40 ug, Primary: Anti-MCP1 (orb704179) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14/11 kD, Observed band size: 11 kD.

Sample: Lane 1: Recombinant rat MCP-1 protein, GST & His, Primary: Anti-MCP1 (orb704179) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 46 kDa.