Cart summary

You have no items in your shopping cart.

CCR-2 Rabbit Polyclonal Antibody

Catalog Number: orb10269

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10269
CategoryAntibodies
DescriptionCCR-2 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityCanine, Equine, Mouse, Rat
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CCR-2 (201-300/373aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW41 kDa
TargetCCR2
UniProt IDP41597
RRIDAB_10747690
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesC-C Chemokine receptor 2B; C C chemokine receptor
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
CCR-2 Rabbit Polyclonal Antibody

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (orb10269), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 80% methanol for 5 min at -20℃ (The cells were fixed with 2% paraformaldehyde for 10 min at room temperature). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

CCR-2 Rabbit Polyclonal Antibody

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (orb10269), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

CCR-2 Rabbit Polyclonal Antibody

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CCR2 Polyclonal Antibody, Unconjugated (orb10269) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • CCR2 Antibody: APC [orb55495]

    ICC,  IF,  IHC

    Human, Rat

    Rabbit

    Polyclonal

    APC

    100 μg
  • CCR2 Antibody: Biotin [orb55496]

    ELISA,  ICC,  IF,  IHC,  WB

    Human, Rat

    Rabbit

    Polyclonal

    Biotin

    100 μg
  • CCR2 Antibody: FITC [orb55497]

    ICC,  IF,  IHC

    Human, Rat

    Rabbit

    Polyclonal

    FITC

    100 μg
  • CCR2 Antibody: HRP [orb55498]

    ELISA,  IHC,  WB

    Human, Rat

    Rabbit

    Polyclonal

    HRP

    100 μg
  • CCR2 Antibody: PerCP [orb55500]

    ELISA,  ICC,  IF,  IHC,  WB

    Human, Rat

    Rabbit

    Polyclonal

    PerCP

    100 μg