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Catalog Number | orb1258366 |
---|---|
Category | Antibodies |
Description | MCAM Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 447-646 of human MCAM (NP_006491.2). |
Concentration | batch dependent |
Dilution range | WB: 1:1000 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 120kDa |
Target | MCAM |
UniProt ID | P43121 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Cell surface glycoprotein MUC18, Cell surface glyc Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of HeLa cells, using MCAM antibody (orb1258366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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