MCAK Antibody

• Catalog Number: orb1529386
• Category: Antibodies
• Description: MCAK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Mouse
• Immunogen: between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate. A300-921A is less efficient at IP than is A300-922A.; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MCAK
• UniProt ID: Q922S8
• NCBI: NP_608301.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Knsl6; X83316; mitotic centromere-associated kines
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse MCAK by immunohistochemistry. Sample: FFPE section of mouse hybridoma tumor. Antibody: Affinity purified rabbit anti-MCAK used at a dilution of 1:1, 000 (1µg/ml).

Detection of mouse MCAK by WB and IP.Samples: NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MCAK antibody orb1529386 used for WB at 0.1 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate (B).