MCAK/KIF2C Antibody

• Catalog Number: orb215984
• Category: Antibodies
• Description: MCAK/KIF2C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81313 MW
• UniProt ID: Q99661
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Kinesin-like protein KIF2C;Kinesin-like protein 6;
• Note: For research use only
• Application notes: WB: The detection limit for MCAK is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Mouse Testis Tissue using Anti-MCAK antibody.

IHC(P) analysis of Rat Testis Tissue using Anti-MCAK antibody.

IHC(P) analysis of Rat Thymus Tissue using Anti-MCAK antibody.

Flow Cytometry analysis of K562 cells using anti-MCAK antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Recombinant Human MCAK Protein 0.5ng using Anti-MCAK antibody.

WB analysis using Anti-MCAK antibody.Lane 1: Mouse Testis Tissue;2:HELA Cell;3: MM231 Cell;4: MM453 Cell;5: SKOV Cell.

IF analysis of MCAK using anti-MCAK antibody. MCAK was detected in immunocytochemical section of U20S cells.