Mast Cell Chymase antibody

• Catalog Number: orb125014
• Category: Antibodies
• Description: Goat polyclonal antibody to CMA1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.5-1 μg/ml, IHC-P: 1.5 μg/ml
• Conjugation: Unconjugated
• MW: 27.3
• Target: CMA1
• Entrez: 1215
• Protein Sequence: QFRHPKYNTSTLHHD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Alpha-chymase antibody, anti Chymase 1 antibo
• Note: For research use only
• Application notes: WB: In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recomm. IHC: Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5µg/ml.. Notes: Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 lysate using Mast Cell Chymase antibody

Immunohistochemical staining of Human Skin using Mast Cell Chymase antibody