CMA1 Antibody

• Catalog Number: orb1248949
• Category: Antibodies
• Description: CMA1 Antibody
• Target: CMA1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-QFRHPKYNTSTLHHD
• UniProt ID: P23946
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CMA1, chymase 1, mast cell, CYH, MCT1, MGC119890,
• Note: For research use only
• NCBI: NP_001827.1

HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with orb1248949 (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1248949 (1 mg/ml) in Lane B.

orb1248949 (1.5 ug/ml) staining of paraffin embedded Human Testis (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248949 (1.5 ug/ml) staining of paraffin embedded Human Tonsil (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.