CMA1 Antibody

SKU: orb1248949

Description

CMA1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1µg/mL.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5µg/mL.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QFRHPKYNTSTLHHD
• Target: CMA1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMA1, chymase 1, mast cell, CYH, MCT1, MGC119890, MGC119891, chymase 1 preproprotein transcript E, chymase 1 preproprotein transcript I, chymase, heart, chymase, mast cell, mast cell protease I

HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with orb1248949 (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1248949 (1 mg/ml) in Lane B.

orb1248949 (1.5 ug/ml) staining of paraffin embedded Human Testis (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248949 (1.5 ug/ml) staining of paraffin embedded Human Tonsil (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CMA1

UniProt

P23946

RefSeq (Protein):NP_001827.1