MAPK1 antibody

• Catalog Number: orb18945
• Category: Antibodies
• Description: Goat polyclonal antibody to MAPK1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 41.4; 41.4
• Target: ERK2 / MAPK1
• Entrez: 5594
• Protein Sequence: CAAGPEMVRGQVF
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MAPK1 antibody, anti ERK2 antibody, anti ERK
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18945 (0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.