MAP2K1/MAP2K2 Antibody

• Catalog Number: orb1258469
• Category: Antibodies
• Description: MAP2K1/MAP2K2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human MAP2K2
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45kDa
• Target: MAP2K1/MAP2K2
• UniProt ID: P36507, Q02750
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of PC-12 cells, using MAP2K1/MAP2K2 antibody (orb1258469) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.