MAP2K1 Antibody

• Catalog Number: orb1273521
• Category: Antibodies
• Description: MAP2K1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Rat
• Immunogen: MEK 1 (Thr292) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr292 of human MEK 1.
• Concentration: batch dependent
• Dilution range: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45
• Target: MAP2K1
• UniProt ID: Q02750
• NCBI: Q02750
• Storage: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
• Note: For research use only
• Application notes: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Expiration Date: 12 months from date of receipt.

Western blots of recombinant wild type and mutant MEK 1 showing phospho-specific immunolabeling. Lanes 1 and 2 are wild type MEK 1; lanes 3 and 4 are mutant MEK 1 (Thr292Arg). MAP kinase was coexpressed in the samples run in lanes 2 and 4.