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Catalog Number | orb1253761 |
---|---|
Category | Antibodies |
Description | MAP2K1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | MEK1 (Phospho-Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 221 (A-N-S (p) -F-V) derived from Human MEK1. |
Concentration | 1 mg/mL |
Dilution range | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 45 kDa |
Target | MAP2K1 |
UniProt ID | Q02750 |
NCBI | NP_002746.1 |
Storage | Store antibody at -20°C for up to one year. |
Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Alternative names | CFC3, MEK1, MKK1, MAPKK1, PRKMK1, EMS1, ERK activa Read more... |
Note | For research use only |
Application notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MEK1 (Phospho-Ser221).
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK1 (Phospho-Ser221).
FC, WB | |
Human, Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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