Mammaglobin A Antibody / SCGB2A2

• Catalog Number: orb2637023
• Category: Antibodies
• Description: Mammaglobin, 10 kDa, is a cytoplasmic protein, a mammary-specific member of the uteroglobin family. It is related to a family of epithelial secretory proteins that includes prostatein and Clara cell protein. Mammaglobin occurs in about 80% of breast carcinomas. The extend is generally larger than that of GCDFP-15. Up to 15% of non-breast carcinomas (such as stomach, lung, colon, hepatobiliary, thyroid, ovarian, and urothelial carcinomas) have been reported positive, usually only focally. Mammaglobin is a sensitive and fairly specific marker for breast carcinoma. The panel should also include Gross cystic disease fluid protein-15 and Estrogen receptor alpha.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rMGB/6619
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human Mammaglobin/SCGB2A2 protein was used as the immunogen for the recombinant Mammaglobin A antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Mammaglobin A antibody is available for research use only.
• UniProt ID: Q13296
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Mammaglobin A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lactating breast tissue with recombinant Mammaglobin A antibody (clone rMGB/6619). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human skeletal muscle with recombinant Mammaglobin A antibody (rMGB/6619) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Mammaglobin A antibody (clone rMGB/6619) as confirmation of integrity and purity.