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Mammaglobin A Antibody / SCGB2A2

Catalog Number: orb2641258

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2641258
CategoryAntibodies
DescriptionMammaglobin, also called Secretoglobin family 2A member 2, or SCGB2A2, is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Species/HostMouse
ClonalityMonoclonal
Clone NumberMGB/2000
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenFull length recombinant human protein was used as the immunogen for the Mammaglobin antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Mammaglobin antibody is available for research use only.
UniProt IDQ13296
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Mammaglobin antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
Mammaglobin A Antibody / SCGB2A2

IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Mammaglobin A Antibody / SCGB2A2

IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Mammaglobin A Antibody / SCGB2A2

Western blot testing of human SK-BR-3 cell lysate (mammary gland tumor cell line) with Mammaglobin antibody (clone MGB/2000). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

Mammaglobin A Antibody / SCGB2A2

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Mammaglobin antibody (clone MGB/2000). These results demonstrate the foremost specificity of the MGB/2000 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Mammaglobin A Antibody / SCGB2A2

SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone MGB/2000) as confirmation of integrity and purity.

Mammaglobin A Antibody / SCGB2A2

IHC testing of FFPE human breast adenocarcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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