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Mammaglobin A Antibody / SCGB2A2

Catalog Number: orb606912

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606912
CategoryAntibodies
DescriptionMammaglobin, also called Secretoglobin family 2A member 2, or SCGB2A2, is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Species/HostRabbit
ClonalityRecombinant
Clone NumberMGB/2682R
Tested applicationsIF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG, kappa
ImmunogenFull length recombinant human protein was used as the immunogen for the recombinant Mammaglobin antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
PurityProtein A affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant Mammaglobin antibody is available for research use only.
UniProt IDQ13296
StorageStore the recombinant Mammaglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the recombinant Mammaglobin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Mammaglobin A Antibody / SCGB2A2

IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10 mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Mammaglobin A Antibody / SCGB2A2

IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10 mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Mammaglobin A Antibody / SCGB2A2

Western blot testing of human HeLa, MCF-7, SK-Br3 and T47D cell lysate with recombinant Mammaglobin antibody (clone MGB/2682R). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

Mammaglobin A Antibody / SCGB2A2

SDS-PAGE analysis of purified, BSA-free recombinant Mammaglobin antibody (clone MGB/2682R) as confirmation of integrity and purity.

Mammaglobin A Antibody / SCGB2A2

Immunofluorescent staining of permeabilized human MCF7 cells with recombinant Mammaglobin antibody (clone MGB/2682R, green) and Reddot nuclear stain (red).

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    IHC-P

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    Rabbit

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