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Catalog Number | orb1957117 |
---|---|
Category | Proteins |
Description | MAG Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56 kDa and the accession number is P20916. |
Tag | C-His |
Purity | 98.00% |
UniProt ID | P20916 |
MW | 56 kDa (predicted); 85 kDa (reducing condition, due to glycosylation) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | MAG Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56 kDa and the accession number is P20916. |
Expression Region | A DNA sequence encoding the human MAG extracellular domain (P20916) (Met 1-Pro 516) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gly 20 |
Storage | -20°C |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.5 kDa after removal of the signal peptide. The apparent molecular mass of MAG-His is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE. | |
28.01 kDa |
98.00% | |
57.6 kDa (predicted). Due to glycosylation, the protein migrates to 78-85 kDa based on Tris-Bis PAGE result. |
> 95% by SDS-PAGE. | |
Recombinant Human MAG/Siglec-4a Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Gly20-Pro516) of human MAG/Siglec-4a (Accession #P20916) fused with a 6��His Tag at the C-terminus. |
98.00% | |
65 KDa (reducing condition) |