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    LMNA antibody

    Catalog Number: orb393095

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb393095
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to LMNA.
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Porcine, Primate, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Lamin A/C. The exact sequence is proprietary.
    Dilution rangeWB: 1:500:1000, IHC-P: 1:100:200
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetLMNA
    Entrez16905, 4000, 60374
    UniProt IDP02545, P48679, P48678
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesLMN1; Prelamin-A/C
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    LMNA antibody

    Western blot analysis of Lamin A/C expression in MCF7 (A), Raw264.7 (B), HepG2 (C) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 74; 65 kD)

    LMNA antibody

    Immunohistochemical analysis of Lamin A/C staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    LMNA antibody

    Immunofluorescent analysis of Lamin A/C staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

    • LMNA antibody [orb48909]

      ChIP,  ICC,  IF,  IHC,  WB

      Human, Mouse, Rat

      Polyclonal

      Unconjugated

      50 μl, 100 μl, 200 μl
    • LMNA Antibody [orb1258730]

      IF,  IHC,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      50 μl
    • Lamin A+C/LMNA Antibody [orb234326]

      FC,  ICC,  IF,  IHC,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      10 μg
    • Lamin A antibody [orb783433]

      ICC,  IHC-P,  WB

      Bovine, Canine, Equine, Porcine

      50 μl, 100 μl
    • Lamin A+C/LMNA Antibody (monoclonal, 5F3C12) [orb1474871]

      IHC,  WB

      Human, Mouse, Rat

      Mouse

      Monoclonal

      Unconjugated

      10 μg, 100 μg
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