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Catalog Number | orb1258730 |
---|---|
Category | Antibodies |
Description | LMNA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human Lamin A / C |
Concentration | batch dependent |
Dilution range | WB: 1:200 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 74kDa |
Target | LMNA |
UniProt ID | P02545 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC, Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (orb1258730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human mammary cancer using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody (orb1254718) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.
ChIP, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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