Lamin A+C/LMNA Antibody

• Catalog Number: orb234326
• Category: Antibodies
• Description: Lamin A+C/LMNA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Mouse Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 74139 MW
• UniProt ID: P02545
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Prelamin-A/C;Lamin-A/C;70 kDa lamin;Renal carcinom
• Note: For research use only
• Application notes: WB: The detection limit for Lamin A/C is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-Lamin A/C antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Lamin A/C using anti-Lamin A/C antibody.Lane 1:human placenta tissue;2:human PC-3 cell;3:human HeLa cell;4:human HepG2 cell.

IF analysis of Lamin A/C using anti-Lamin A/C antibody. Lamin A/C was detected in immunocytochemical section of A431 cells.

IF analysis of Lamin A/C using anti-Lamin A/C antibody. Lamin A/C was detected in immunocytochemical section of NIH3T3 cells.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody. Lamin A/C was detected in a paraffin-embedded section of Mouse Intestine tissue.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody. Lamin A/C was detected in a paraffin-embedded section of Human Mammary Cancer tissue.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody. Lamin A/C was detected in a paraffin-embedded section of Rat Intestine tissue.