LGALS3 Antibody

• Catalog Number: orb1274508
• Category: Antibodies
• Description: LGALS3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 31kDa
• Target: LGALS3
• UniProt ID: P17931
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Gale
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using LGALS3 antibody (orb1274508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (orb1274508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of C6 cells using LGALS3 antibody (orb1274508) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L-929 cells using LGALS3 antibody (orb1274508) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using LGALS3 antibody (orb1274508) at dilution of 1:100. Blue: DAPI for nuclear staining.