Galectin 3/LGALS3 Antibody

• Catalog Number: orb196251
• Category: Antibodies
• Description: Galectin 3/LGALS3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26152 MW
• UniProt ID: P17931
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-bindin
• Note: For research use only
• Application notes: WB: The detection limit for Galectin 3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-Galectin-3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Galectin-3 using anti-Galectin-3 antibody.Lane 1:human HeLa cell; 2:human SiHa cell; 3:human U87 cell; 4:human Hacat cell; 5:human PC-3 cell; 6:human T47D cell.

IF analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in immunocytochemical section of U20S cell.

IF analysis of Galectin-3 using anti-Galectin-3 antibody. Galectin-3 was detected in an immunocytochemical section of SiHa cells.

IHC analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in paraffin-embedded section of Human Lung Cancer Tissue.

IHC analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in paraffin-embedded section of Human Placenta Tissue.