Galectin 3/LGALS3 Antibody (monoclonal, 12B12)

• Catalog Number: orb527053
• Category: Antibodies
• Description: Galectin 3/LGALS3 Antibody (monoclonal, 12B12)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12B12
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2a
• Immunogen: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 28 kDa
• UniProt ID: P17931
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-bin
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-Galectin 3/LGALS3 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-Galectin 3/LGALS3 antibody.Lane 1:HeLa cell;2:U87 cell;3:PC-3 cell.4:human T47D cell;5:human CACO-2 cell;6:human placenta tissue;7:human A549 cell;8:human HL-60 cell;9:rat lung tissue;10:rat testis tissue;11:mouse lung tissue.

IF analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in immunocytochemical section of A431 cells.

IF analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of human melanoma tissue.

IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of rat small intestine tissue.