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Catalog Number | orb1247948 |
---|---|
Category | Antibodies |
Description | LEPR Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Canine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-TQDDIEKHQSDAG |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration:0.1-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Heart. Recommended concentration: 4ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | LEPR |
UniProt ID | P48357 |
NCBI | NP_001003679.1, NP_002294.2, NP_001003680.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | LEPR , leptin receptor , CD295, OBR , OB receptor, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration:0.1-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Heart. Recommended concentration: 4ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247948 (1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247948 (4 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247948 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
ELISA, FC, IHC, WB | |
Canine, Human | |
Goat | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Primate, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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